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KMID : 0364819870250010052
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Korean Journal of Microbiology
1987 Volume.25 No. 1 p.52 ~ p.56
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Studies on the Receptor for Bacteriophage N4 Infection
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채건상/Chae, K.S
김선정/김창수/유욱준/Kim, S.J./Kim, C.S./Yoo, O. Joon.
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Abstract
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1
The evidences that lam B protein of E. coli is used as a receptor for infections of bacteriophage N4 as well as bacteriophage lambda were obtained from the following experimental results. First, all of the isolated lambda resistant clones possessing foreign DNA fragments in the plasm ids were also resistant to bacteriophage N4, but not to bacteriophage 080, T4 and T7. Second, when the plasmid DNA was treated with various restriction enzymes and ligated to delete the total or a portion of the foreign DNA fragments, the deleted plasmids lost the resistant activities to lambda and N4, simultaneously. Third, after amplification of Lam B protein about 200 times by inducing the protein using maltose as a sole carbon source, the host E. coli became sensitive to both lambda and N4.
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KEYWORD
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